Laboratory of New and little explored Infection
List of research projects carried out in the laboratory 2001-2004
 

 

1. Name of research project: “The development a express diagnostic and treatment control method (polymerase chain reaction based) for legionella infection”.

Project start and accomplishment data: 2002.01-2004.12.

Project financing: Academy of medical Science Ukraine.

The collaborating organization: LtdBioanalytical technolohies” “B.A.T.” (Kharkov, UA), E-mail: bat@kharkov.com

Head of the project: Pokhil S.I., Director Scientific IMI, Head of LNLEID, Ph. D., Principal Scientist; Shagun S.V., Director Ltd “B.A.T.”.

 

2. Name of research project: “Effect of laser radiation on biological properties (expression of the pathogenic factors) of the agents of infectious diseases”.

Project start and accomplishment data: 2001.01-2003.12.

Project financing: Joint-Stock CompanyCenter of Laser and Microwave Technologies” (Kharkov, UA).

The collaborating organization:Research Institute for Laser Biology and Laser Medicine, Kharkov State University (RILBLM).

E-mail: Laser@VL Kharkov.ua; URL: http://www.medlaser.com.ua.

Head of the project: Pokhil S.I., Director Scientific IMI, Head of LNLEID, Ph. D., Principal Scientist; Korobov A.M., Director RILBLM, Ph. D.

List of current research projects (2005) opened to collaboration:

 

1. Name of research project: “Stady of biological properties of Bartonella spp. and development of laboratory diagnostics methods for cat-scratch disease”.

Project start and accomplishment data: 2005.01-2007.12.

Project financing: Academy of medical Science Ukraine.

Head of the project: Pokhil S.I., Director Scientific IMI, Head LNLEID, Ph. D., Principal Scientist

 

 

Head of DepartmentSergey I. Pokhil


Staff
:

 

Sergiy I. Pokhil, Head of LNLEID, Senior Research Scientist, Ph.D. of medicine

Sc. interests:

Ph.D. Thesis: ”Lysogeny, bacteriocynogenity, some factors of pathogenicity in Klebsiella: their applied significance” (1992).

Work address: Mechnicov Institute of Microbiology and Immunology, 14/16, Pushkinskaya Str., Kharkov, 61057, Ukraine

Olena N. Timchenko, Research scientist, Ph.D. of medicine

Sc.interests: molecular biology, DNA restriction patterns, epidemiological genotyping of bacteria.

Ph.D. Thesis: ”An improvement of molecular-genetic methods of intraspecific epidemiological typing of clinically significant microorganisms from different taxonomic groups” (2005).

Valeriy V. Krasovsky, Research scientist, Ph.D. of medicine

Sc.interests: clinical microbiology, method of determination of microorganisms viabilities, methods of determination of desinfectants effect.

Ph.D. Thesis: ”Biological properties of bacteria of Listeria species circulating in Ukraine” (1995).

Nila A. Chigirinskaya, research scientist

Yelena A. Repnia, assistent

Oleg V. Beznoschenko, assistent

Tatyana A. Kruglova, assistent

•Natalia N. Konoval, laborant

Olena N. Musatova, laborant

Main publications:

1.Pokhil S., Smelanskaya M. Method of sample preparation for electron microscopy study of fibriae structures of bacteria// Klinikal and laboratory diagnostics.1993;(5);22-24

2.Tsiganenko A., Pokhil S. Methods of bacteria classifications: modern state of the problem// The medicine problem bulletin.1995;(9);15-24

3.Tsiganenco A., Pokhil S. Thoughts on modern classification and nomenclature for bacteria of Salmonella species// The medicine problem bulletin.1995;(10);89-100

4.Krasovsky V., Volansky Yu., Pokhil S. Characteristics of biological properties of Listeria strains isolates in Ukraine and Belgium// Microbiol.J.1996;58(3),10-17 5.Pokhil S. Species of enterobacteria new to medicine// Microbiol.J.1996;58(3);94-103.

6.Pokhil S., Korniyenko I., Pokhil Sv. Biological properties and medicinal significance of Rahnella aquatilis// Bulletin of medicine and biology problems.1996;(4);129-140

7.Pokhil S. Rarely occuring, atypical and new enteerobacteria// Microbiol.J.1996;58(4);93-104

8.Tsiganenko A., Pokhil S. To 100-th anniversary of the discovery of Widal's reactions of agglutination Chistory of serodiagnostics development// Microbiol.J.1996;58(5);107-109

9.Pokhil S. The biological properties of adsorbed and nonadsorbed OH-agglutinating sera for the identification of Rahnella aquatilis bacteria// Microbiol.J. 1997;59(1);70-76

10.Pokhil S., Mackiy N. Criteria of etiological diagnostics of the acute intestinal infections caused by Rahnella aquatilis// Infecious Diseas.1997;(3).10-13 11.Pokhil S. Bacteriological diagnostics of diseases caused by Rahnella aquatilis// Clin.Lab.Diag.1997; (8); 44-47

12.Timchenko Ye., Volansky Yu., Pokhil S. Analysis of restriction patterns of DNA as a method of modern epidemiological typing of the pathogenic agents of infection diseases// Bulletin of medicine and biology problems.1997;(32);78-86 13.Pokhil S., Savin R. Application of divice "Pulse-Gep1"for conducting gel-electrophoresis in the pulsatins field// Ukrainian Journal of Medical Equipment and Technology.1997;(3-4);71-75.

14.Pokhil S., Pokhil Sv. Intestinal CLV-infection in the Ukraine// Infections Diseases.1998;(1);10-14

15.Tsiganenko A., Timchenko Ye., Pokhil S. DNA restriction patterns as a modern method of bacteria classification// Bulletin of medicine and biology problems.1998;(2);16-26

16.Pokhil S., Krasovsky V., Timchenko Ye. Assessing the weight of inoculate applied by Semenikhina's replicator// Clin.Lab.Diag.1998;(7);34-36

17.Pokhil S. Biological properties of Rachnella aquatilis strains isolated in different regions// Microbiol.J.1998;60(3);31-36.

18.Pokhil S., Derkach S., Pivnenco T., Pertskhalava T. Identification of species of Enterobacteriace family producing hydrogen sulphoxide//Microbiol.J.998;60(4);3-14

19.Pokhil S.I. Properties of Pragia fontium bacteria isolated in the territory of Ukraine and Czechia// Microb.Z. (Ukraine). 2000; 62(2); 3-10

20.Krasovsky V.V., Vasilyev N.V., Derkach S.A., Pokhil S.I. Results of the five-year study of listeriosis in the Ukraine// Zh. Microbiol. (Moscow). 2000;(3); 80-85

21.Krasovsky V.V., Nadtoka V.L., Pokhil S.I. Listeria carriership: a sanitation method// Zh. Microbiol. (Moscow). 2000;(3); 18-20

22.Limansky A.P., Limanskaya O.Yu., Belozersky V.I., Timchenko Ye.N., Pokhil S.I. Development and trials of polymerase chain reaction for detection of meningococcal infection agent// Clin. Lab. Diag. (Moscow). 2000;(4); 44-47 23.Krasovsky V.V., Pokhil S.I., Limansky A.P., Limanskaya O.Yu., Timchenko Ye.N. Development and trials of polymerase chain reaction for detection of Listeria// Clin. Lab. Diag. (Moscow). 2000;(6); 37-41

24.Pokhil S.I. Evolutionary Phylogenetic Classification of Infections Diseases. - Kharkov, 2000. - 27 p.

25.Pokhil S.I. Microbiological characteristics of Rahnella aquatilis srtains isolated from patients with diarrea. Vesnic Sum. State Univ. 2001. 32 (11); 132-137. 26.Timchenko Ye.N. Pheno- and genotypic of Staphylococcus aureus// Vestnik Sum. State Univ. 2001; 32(11); 143-147

26.Volyanskaya N.P., Grechko V.A. Some tendencies of chemotherapy and chemoprophylactics of acute inflammatory diseasces in modern clinics// Vestnik Sum. State Univ. 2001; 32(11); 106-113

27.The method of isolation, identification and longtermin deposition of pure cultures of Rahnella aquatilis/ Pokhil S.I., Osolodchenko T.P., Volyanska N.P. et al. In : “Methods of recieving of pure microorganisms cultures and of their long-time saving in collections: Works of the stuff of the museum of microorganisms to be pathogenetic for human”. - Kiev: Znannya, 2000. - P. 66-80

28.The method of isolation, identification and longtermin deposition of pure cultures of Listeria/ Krasovsky V.V., Pokhil S.I., Osolodchenko T.P. et al. In : “Methods of recieving of pure microorganisms cultures and of their long-time saving in collections: Works of the stuff of the museum of microorganisms to be pathogenetic for human”. - Kiev: Znannya, 2000. - P. 49-65

29.Culture catalogue: Bacteria. Mycoplasma. Chlamydia. Yeasts-like fungi. Mycelious fungi. Cell Cultures/ Selnikova O.P., Mavrov I.I., Boschenko Yu.A., Pokhil S.I., - Kiev: Znannya, 2000. - 119 p

30.Timchenko Ye.N. Genotypic of hospital strains of Staphylococcus aureus by analysis of the length polymorphism of restrictions of chromosome DNA// Vestnic Khar. State. Univ. 2004;(2); 72-75

31. Tsyganenko A.Ya., Pokhil S.I., Timchenko O.M. Fasks and ways of further improvement genotyping infections diseases exciters by analysis polymorphism of fragments length restriction endonuclease of genomic DNA// Experimental and clinical medicine. 2002; (3); 79-83

32. Maryuschenko A.M., Volyanskaya N.P., Pokhil S.I. Research of rahnellous infection exciter’s sensitinty to antibacterial preparations// Experimental and clinical medicine. 2002;(3); 83-87

33. Pokhil S.I. The study of environments of the vital activity of Budvicia aquatica bacteria on territory of the Ukraine. Biological properties of B. aquatica strains isolated on territory of the Ukraine and Czechia// Annals of the Mechnikov  Institute. 2002; (2-3); 20-26

34. Kozko V.M., Krasovsky V.V., Volianskaya N.P., Maryuschenko A.M., Pokhil S.I. The estimation of the spreadind extent of Listeria pathogenic forms amongst epidemiologically endangered professional groups of people. Sanitation of Listeria carriers with chlorophyllipt// Annals of the Mechnikov Institute. 2003;.(4-5); 58-59

35. Varbanets L.D., Ostapchuk A.N., Pokhil S.I. Characteristics of structure components of Rahnella aquatilis lipopolysaccharides// Microb. Z. (Ukraine).2004; 66(4); 13-21

36. Smelianskaya M.V., Pokhil S.I. The place of cytomegalovirus amongst infectious agents isolated from sperm// Dermatology and Venereology. 2001; 1(11); 70-72

37. Tsyganenko A.Ya., Minukhin V.V., Pokhil S.I. Time to change dogmas. Modern theory and practise of discovering new pathogen agents of infectiuos disease. –Kharkiv, 2005. –48 p.

38. Martynov A.V., Timchenco O.M., Pokhil S.I. Chemically modified specific immunoglobulins for legionelliosis laboratory diagnostics// Microb. Z. (Ukraine). 2005; 67(3); 61-70

39. Martynov A.V., Timchenco O.M., Pokhil S.I. Chemical modification for rising lysing activity of bacteriophages// Microb. Z. (Ukraine).-2005. –Vol.67   . –Ð.